Steps for the dissection of male spodoptera moths lepidoptera. Mediterranean brocade, spodoptera littoralis boisduval, 1833, noctuidae nattflyn. An egyptian isolate of spodoptera littoralis nucleopolyhedrovirus splinpv was tested for its potential as biocontrol agent in comparison to autographa californica multiple nucleopolyhedrovirus acmnpv. Spodoptera littoralis valent biosciences biorational crop. A new website is available for this genome, test it here. The laboratory strain was reared in the laboratory of sakha agricultural research station on castor bean leaves. Soil drenching with entomopathogenic fungi for control of the soildwelling life stages and adults of the same generation of spodoptera littoralis boisd. The cotton leafworm, spodoptera littoralis boisd is one of the major insect pests that cause a considerable damage to many of the important vegetables and field crop in egypt. Spodoptera eridania, spodoptera frugiperda, spodoptera littoralis, and spodoptera litura lepidoptera.
Pesticide formulations as a key factor affecting chlorpyrifosethyl and lambdacyhalothrin performance against spodoptera littoralis boisd. Essential oil monoterpenoid on spodoptera littoralis journal of biopesticides, 33. Jan 23, 2008 an egyptian isolate of spodoptera littoralis nucleopolyhedrovirus spli npv was tested for its potential as biocontrol agent in comparison to autographa californica multiple nucleopolyhedrovirus ac mnpv. Spodoptera littoralis spodoptera litura larval diagnosis detailed diagnosis of mature larval spodoptera was given in the fact sheet for s. The purpose of this handout is to guide you in the dissection, screening, and identification of moths collected in spodoptera surveys. Establishment and characterization of three new embryonic. Notes on taxonomy and nomenclature top of page for about 60 years, the egyptian cotton leafworm was known as prodenia litura. The present study scrutinised how far temperature would affect the velocity of the insecticidal activity of bacillus thuringiensis, as the rapidity of pest control achievements is of a great concern. Spodoptera littoralis georgia invasive species task force.
Extensive host plant range including ornamentals, vegetables, fruits and row crops. M eagher 1, j ulieta b rambila 2 and e dward h ung 3 1 usdaars center for medical, agricultural and veterinary entomology, 1700 sw 23 rd drive, gainesville, fl 32608 2 usdaaphisppq, p. Ac, the compounds are loaded into the lure dispensers in different amounts depending on the target species. Because of the extensive and continuous use of traditional insecticides, this led to the development of resistance in this insect, so there is a constant demand to use new control agents. Transcriptome analysis of gene families involved in. Development and validation of realtime pcr tests for the. Adults feed on nectar, and females oviposit on the leaves of crop. The panel on plant health undertook a pest categorisation of. The laboratory strain of spodoptera littoralis was provided from the division of cotton leafworm of plant protection research institute, dok ki, egypt. For the in vitro production of splimnpv a cellular system has to be established. Multiplication of spodoptera littoralis granulosis virus in a. Spodoptera littoralis multicapsid nucleopolyhedrovirus splimnpv could thus be applied to protect plants against the african cotton leafworm. Synergistic activity of a bacillus thuringiensis delta.
The study of olfaction is key to understanding the interaction of insects with their environment and provides opportunities to develop novel tactics for control of pest species. The ricecotton cutworm spodoptera litura is a polyphagous pest of over 100 different host plants. Epas registered antimicrobial products for use against novel coronavirus sarscov2, the cause of covid19 author. Candidate chemosensory genes in female antennae of the. The egyptian cotton leafworm, spodoptera littoralis, a destructive insect pest for a wide range of host plants. March 15, 2014 biology and ecology spodoptera littoralis larvae damage many agricultural plants, particularly cotton venturini, 1975.
Pm 7124 1 spodoptera littoralis, spodoptera litura. Pm100228 eppo a1 and a2 lists of pests recommended for regulation as quarantine pests 2019. Positive identification requires dissection of adult. Flight response of spodoptera littoralis females and males to sprayed cotton headspace, and synthetic blends using a piezoelectric sprayer in a flight tunnel assay. Le ver du cotonnier spodoptera littoralis boisduval. The cotton leafworm, spodoptera littoralis, is a phytophagous pest and current focal species for insect chemical ecology and neuroethology. Low predation rates were achieved by tethering the moths on a short 20 cm piece of cotton thread directly to the plants and.
Spodoptera littoralis is native to africa, the middle east and the mediterranean countries of europe. It seems like the decision by the female on how many eggs to oviposit could be controlled at two levels. Media in category spodoptera littoralisthe following 20 files are in this category, out of 20 total. Use of dna barcodes to identify invasive armyworm spodoptera species in florida rodney n. Heritability of immune function in the caterpillar. Several noctuid genera such as elaphria, galgula, spodoptera wagner et al. The egg masses were collected daily and they were hatched on the oleander leaves. Insecticidal effects of pure and silverdoped copper oxide. The results suggest amino acid substitution in open reading frame of phantom, shade and disembodied protein in spodoptera litura. This moth is found in asia, with some specific problematic pest population reports occurring in cambodia, hong kong, india, the pacific islands, guam, american. Pdf effects of the spodoptera littoralis granulovirus on.
Microbial control of the cotton leafworm spodoptera littoralis boisd. Comparative assays of spli npv and ac mnpv against 2nd instar larvae of spodoptera littoralis revealed 4fold greater susceptibility of s. This page was last edited on 10 december 2014, at 19. Spodoptera littoralis spodlioverview eppo global database. Headspace was released at 1800 ngh of the main compound dmnt. This discussion focuses on early instar spodoptera and those spodoptera that can only be identified to genus. All structured data from the file and property namespaces is available under the creative commons cc0 license. Adults can live for 8 to 10 days eggs hatch in 4 to 12 days larvae pass through six instars in about days after the mature larval stage, pupation takes place. The purpose of this handout is to guide you in the dissection, screening, and identification of moths collected in spodoptera. Effect of sublethal doses of some insecticides and their.
Spodoptera species uk plant health information portal. A fast and simple method of preparing virgin females of spodoptera littoralis boisd. We identified the sequence of five halloween genes partially and converted into amino acid sequences and then three of them were compared with a very near african species of spodoptera, spodoptera littoralis. Spodoptera littoralis can be distinguished from native species using genitalic characters. Michigan state universitys invasive species factsheets. These two species are hard to discriminate between because the larvae and adult forms are identical. Spodoptera species 517 monitoring for exotic spodoptera species lepidoptera.
The role of temperature in enhancing the insecticidal. Spodoptera littoralis, also referred to as the african cotton leafworm or egyptian cotton leafworm or mediterranean brocade, is a species of moth in the family noctuidae. Moth photographers group spodoptera littoralis 19640. Spodoptera litura is also known as the oriental leafworm moth, cluster caterpillar, cotton leafworm, tobacco cutworm, tropical armyworm, taro caterpillar, tobacco budworm, rice cutworm, and cotton cutworm. During heavy infestations, however, they can also penetrate flowers and bolls. Field key to late instars of spodoptera of america north of mexico steven passoa the following key, based on levy and habeck 1976, passoa 1991, godfrey 1987, and pogue 2002, will facilitate the identification of ten spodoptera species known to. Since the species is nocturnal, its peak flight activity is usually between the hours of 20. How to distinguish some native species spodoptera dolichos one of the characters that helps to distinguish s. The ricecotton cutworm generalist plantfeeding moth. It has been recorded several times in the uk, and even though it has been intercepted at u. We would like to show you a description here but the site wont allow us. It is a highly polyphagous organism that is a pest of many cultivated plants and crops. Meagher1c 1center for medical, agricultural and veterinary entomology, usda ars, gainesville, fl 32608 2entomology, usda.
Here we use transcriptome and expression profiling. Files are available under licenses specified on their description page. Some authors mistakenly continue to regard the allopatric old world cotton leafworm species s. Spodoptera littoralis valent biosciences biorational. Noctuidae is one of the most destructive agricultural pests. Spodoptera littoralis wikipedia, a enciclopedia livre.
Gooch as a part of michigan state university ipm program and m. Effect of hyptis brevipes lamiaceae methanol extract on spodoptera littoralis lepidoptera. A technique for tethering females of spodoptera littoralis. Development and validation of realtime pcr tests for the identification of four spodoptera species. Spodoptera littoralis, also known as the african cotton leafworm, egyptian cotton leafworm or the mediterranean brocade, is native to africa and israel and widely found in both africa and the mediterranean europe. Frontiers flight attraction of spodoptera littoralis. Spodoptera littoralis boisduval for the territory of the european union eu. Recent developments in transcriptomic approaches enable the molecular basis of olfaction to be studied even in species with limited genomic information.
Noctuidae is one of the key pests that cause great damage to cotton plants as well as other plants in egypt willcocks and bahgat, 1937, larvae of this pest can feed on. Growth disruption, abnormalities and mortality of spodoptera. In spain, italy and greece the insect can be found in numerous greenhouse crops including tomato, pepper and melon. Eggs are somewhat flattened, spherical, and laid in clusters with a felt.
Keys about fact sheets glossary larval morphology references. Pdf converter is a online webbased document to pdf converter software. Heritability of immune function in the caterpillar spodoptera littoralis sc cotter and k wilson institute of biological sciences, university of stirling, stirling fk9 4la, 11k npt phenoloxidase po is believed to be a key mediator of immune function in insects and has been implicated both in nonself recognition and in resistance to a variety of. General information about spodoptera littoralis spodli eppo global database. Spodoptera littoralis boisduval is an important pest of cotton in southern europe, africa and the middle east hosny et al. We investigated the cumulative lethal effects of two nanostructure materials by feeding nanostructural form of copper oxide cuo or copper oxide doped with silver cuo. Documents about spodoptera littoralis spodli number title download.
The cotton leafworm, spodoptera littoralis boisduval lepidoptera. Ag, nanosheetstreated diet to third andor fifth instars of s. Convert and create pdf from various types of files like word doc, excel xls, powerpoint ppt. Gray circles indicate which compounds are included in each blend. Noctuidae and some native spodoptera moths introduction the purpose of this handout is to help you distinguish two exotic spodoptera moths of concern to u. Occurrence, abundance, and efficiency of some local. Data on mandible morphology was taken from brown and dewhurst 1975 african species, tanada and beardsley 1957 s. Sabry abstract toxicity of the two biorational insecticides, spinosad and buprofezin and a mixture of the two was tested against the fourth instar larvae of the cotton leafworm, spodoptera littoralis boisduval. Pdf soil drenching with entomopathogenic fungi for. Induced plant volatiles and their effect on spodoptera. Pholidoptera littoralis is a species belonging to the family tettigoniidae subfamily tettigoniinae. Feeding and life history of spodoptera exigua lepidoptera. Occurrence, abundance, and efficiency of some local parasitoids attacking spodoptera littoralis lepidoptera.
This proprietary strain of bacillus thuringiensis aizawai abts1857 contains two additional toxin proteins with very good, highlyspecific activity against spodoptera species. We produced male and female illuminabased transcriptomes from chemosensory and nonchemosensory tissues of s. Inspecting the genitalia is the most certain way to tell the two species apart. The egyptian cottonworm is a highly polyphagous defoliator of many cultivated plants. The following key is designed to help separate middle to late instar s. Simplified field key to late instars of spodoptera of america. More than 100 species of host plants are reported, many of which are of economic importance eppocabi, 1997, robinson et al.
View spodoptera littoralis research papers on academia. Spodoptera littoralis michigan state universitys invasive species factsheets prepared by t. For this purpose three new continuous cell lines were established from embryonic tissues of s. Noctuidae on different host plants article pdf available in annals of the entomological society of america 94jul 2001. It is found from greece across much of the balkan peninsula on to the italian alpine foothills, switzerland and austria to germany, the czech republic, poland and slovakia, where it is less common. Spodoptera littoralisis a destructive pest of subtropical and tropical agriculture, and has the potential to be a serious pest of glasshouse crops in northern europe. Comparison of amino acid sequences of halloween genes in. Characterization of an egyptian spodoptera littoralis nucleopolyhedrovirus and a possible use of a highly conserved region from polyhedrin gene for nucleopolyhedrovirus detection alaaeddeen m seufi 1 virology journal volume 5, article number. Gooch as a part of michigan state university ipm program and.
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